Antioxidant, chelating and HPLC quantification of phenols in extract of Phyllanthus niruri
Department of Biochemistry, Niger Delta University, Amassoma, Bayelsa State, Nigeria.
Research Article
World Journal of Advanced Pharmaceutical and Life Sciences, 2022, 03(01), 001–006.
Article DOI: 10.53346/wjapls.2022.3.1.0029
Publication history:
Received on 28 June 2022; revised on 04 August 2022; accepted on 06 August 2022
Abstract:
Phyllanthus niruriis a known medicinal plant. The leaves of Phyllanthus niruri were evaluated for antioxidant activity utilizing different methods and the quantification of phenols and flavonoid using HPLC (High Performance Liquid Chromatography). The leaves of Phyllanthus niruri were harvested, shade dried, grounded and extracted with methanol. The extract was subjected to total antioxidant capacity, DPPH radical scavenging, nitric oxide (NO) scavenging, ferrous chelation and HPLC quantification of phenolics and flavonoids. The result showed total antioxidant capacity of 13.04 ±0.08 mgAAE/g. The % scavenging of DPPH radical of gallic acid a standard antioxidant was slightly higher than Phyllanthus niruri at concentrations of 0.1 – 1 mg/ml. In the case of nitric oxide (NȮ), the scavenging ability of Phyllanthus niruri was higher than the standard antioxidant quercetin at 0.1 – 1 mg/ml. the chelating ability of Phyllanthus niruri and EDTA at the same concentrations of 0.1 – 1 mg/ml shown in table 3 shows that Phyllanthus niruri has about 70 % of the chelating ability of EDTA. Some important phenols and flavonoids detected in Phyllanthus niruri are P-coumaric acid, gallic acid, caffeic acid, kaempferol, ferulic, luteolin, quercetin amongst others. This investigation shows that extract of Phyllanthus niruri acts as an antioxidant due to the abundance of phenolics and flavonoids.
Keywords:
Phyllanthus niruri; Phenols; Flavonoids; Antioxidant; Chelating
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