The effect of resveratrol and alcohol on zebrafish caudal fin regeneration
Department of Basic Medical Science, Faculty of Medical Sciences, University of West Indies, Mona campus, Kingston, Jamaica, West Indies.
Research Article
World Journal of Advanced Pharmaceutical and Life Sciences, 2024, 06(02), 017–022.
Article DOI: 10.53346/wjapls.2024.6.2.0031
Publication history:
Received on 10 May 2024; revised on 22 June 2024; accepted on 25 June 2024
Abstract:
Introduction: Wine, an alcoholic beverage derived from the fermentation of fruit juices, typically grapes, has been a popular drink since ancient times. Resveratrol, a polyphenol found in grapes, is associated with growth inhibition. There are conflicting reports on the overall health benefits of regular wine consumption, with some sources suggesting positive effects on growth, while others claim inhibitory effects. This study investigates the effects of red wine, grape juice, and resveratrol on growth and wound healing using adult zebrafish.
Method: The study assessed growth through the regeneration of the caudal fin (CF) in adult zebrafish. The CF was amputated, and the fish were subjected to various treatments: different concentrations of alcohol, grape juice (GJ), resveratrol, red wine, and combinations of alcohol with grape juice (A+GJ) or resveratrol (A+RV). Treatments were administered for 1 hour, three times daily, with a 1-hour recovery period between treatments, over 14 days. CF growth was measured using Fiji ImageJ.
Results: Grape juice, alcohol, and red wine treatments resulted in significant, concentration-dependent reductions in CF regeneration compared to untreated fish. The A+GJ combination showed increased regeneration compared to GJ alone, but regeneration was still lower than in untreated fish. Resveratrol-treated fish exhibited higher CF regeneration compared to the control. High concentrations of resveratrol in A+RV treatments caused significant toxicity, while lower doses led to reduced CF regeneration.
Conclusion: The growth inhibition observed with red wine and GJ treatments may not be solely due to resveratrol but rather the interaction of resveratrol with other components of GJ and/or alcohol.
Keywords:
Growth; Red wine; Grapes; Alcohol; Zebrafish
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