GC-MS bioactive compounds in Ocimum gratissimum methanol and hexane extracts a comparative study
Department of Biochemistry, Niger Delta University, Amassoma, Bayelsa State, Nigeria.
Research Article
World Journal of Biological and Pharmaceutical Research, 2022, 02(02), 076–081.
Article DOI: 10.53346/wjbpr.2022.2.2.0034
Publication history:
Received on 25 April 2022; revised on 03 June 2022; accepted on 05 June 2022
Abstract:
Bioactive compounds in medicinal plants are very important because they are the active ingredients in the plant. Therefore this work is aimed at determining the bioactive compounds in methanol and n-hexane extracts of Ocimum gratissimum using gas chromatography mass spectrometry (GC-MS). The leaves of Ocimum gratissimum were dried and grounded and extracted with methanol and n-hexane separately. The extract was concentrated to a dark paste, which was later subjected to GC-MS analysis. Results revealed the presence of many compounds for example octacosane with peak number 47 retention time RT 22.521 and % area of 7.82, other extracts of Medicinal importance are 2,6,10,14,18,22-tetracosahexaene RT 24.584 area % 5.51 and peak number 55, erythro-9,10-dibropentacosane with a peak number 52 RT 24.142 and % area 3.30, heneicosane has a peak number 43, RT 22.024 and % area 5.45, 2-methyloctacosane has a peak number 36 RT 20.069 and area % of 5.57.
Keywords:
GC-MS; Ocimum Gratissimum; Peak Number; Retention Time and % Area
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