Isolation of a vasodilator molecule from Moringa oleifera L. (MORINGACEAE) seeds using bioassay‐guided fractionation
1 Department of Pharmacognosy and Herbal Medicine, University of Ghana, Box LG 610, Legon Accra, Ghana.
2 Department of Chemistry, Science Faculty, University of Antsiranana, Madagascar.
3 Department of Pharmacology, Faculty of sciences, University of Antananarivo, Madagascar.
4 Department of Chemistry, Faculty of sciences, University of Antananarivo, Madagascar.
Research Article
World Journal of Chemical and Pharmaceutical Sciences, 2025, 07(01), 009-016.
Article DOI: 10.53346/wjcps.2025.7.1.0026
Publication history:
Received on 06 November 2025; revised on 15 December 2025; accepted on 18 December 2025
Abstract:
This work aimed to identify a vasodilator molecule from Moringa oleifera (MORINGACEAE) seeds. Bio guided fractionating was carried out to identify the most effective fraction, and the active molecule was isolated from the most effective fraction. Reflux extraction with solvents of increasing polarity was carried out. Tested on isolated aorta, ethyl acetate and methanolic fractions exhibited vasodilation activity. They were injected cumulatively into a bath containing aorta pre contracted with norepinephrine at 10-5 M. Results indicated that both extracts relax the isolated organ in a concentration effect dependent manner, with respective EC50 of 1.06 and 2.63 mg/ml (p < 0.05). These results indicate that ethyl acetate extract exhibits more potency. An active molecule was isolated from ethyl acetate fraction using preparative chromatographic plate techniques and its structure was established by spectroscopic techniques (UV, 1D- and 2D NMR, MS). The results showed 6 spots from ethyl acetate fraction with respective retention factor (Rf) of 0.13, 0.22, 0.24, 0.25, 0.45 and 0.54 when visualized under UV light at 254 nm. Tested on isolated aorta pre contracted with norepinephrine at 10-5M, the molecule with Rf 0.13 relaxes the isolated aorta with EC50 of 9.82.10-5 M. Chemical shifts (δH) and spin-spin coupling constants (JHH) data were compared with literature to confirm the isolate as (N-ethyl-3-((3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yl)oxy)pent-4-enamide, belonging to the group of amides.
Keywords:
Vasodilator; Isolated Aorta; N-428; Moringa oleifera
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