Pipetting sample preparation with water eluent followed by water mobile phase HPLC analysis for residual monitoring of melamine in milk
Graduate School of Human Life and Ecology, Department of Nutrition, Osaka Metropolitan University, Osaka 558-8585, Japan.
Research Article
World Journal of Chemical and Pharmaceutical Sciences, 2023, 02(01), 017–022.
Article DOI: 10.53346/wjcps.2023.2.1.0032
Publication history:
Received on 20 November 2022; revised on 10 January 2023; accepted on 13 January 2023
Abstract:
The author introduces a small-scale, time shortening, economical sample preparation with water eluent followed by an isocratic water phase HPLC system for quantifying melamine in cow’s milk. Sample preparation is achieved by homogenization using a handheld ultrasonic-homogenizer with water followed by MonoTipⓇC18 pipette tip contains silica monolith bonded with octadecyl group with water eluent. For determination and identification of analyte, the HPLC uses an analytical C18 column, an isocratic 100% water, and a photo-diode array detector. The procedure, performed under a 100% water conditions, uses no organic solvents and poisonous reagents at all and is, therefore, harmless to both humans and the environment. The method validation data were well within the international method acceptance criteria. The total analytical time and quantitation limit were < 6 min/sample and 0.24 μg/mL, respectively. The present technique may be proposed as an international harmonized analytical method for routine residue monitoring for melamine in milk.
Keywords:
International harmonized analytical method; Pipette tip; Water mobile phase HPLC; Melamine; Residue monitoring
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0