Analysis of Active Components and High-Performance Liquid Chromatography (HPLC) Profiling of Emperor Scorpion (Pandinus imperator) against Selected Bacterial Strains
1 Department of Microbiology, Faculty of Science, University of Abuja, Nigeria.
2 Department of Biochemistry, Faculty of Science, University of Abuja, Nigeria.
3 Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Abuja, Nigeria.
* Corresponding Author
ORCID Details
Ayodeji Adams DAHUNSI: https://orcid.org/0009-0007-5025-9482
Bukola Catherine AKIN-OSANAIYE: https://orcid.org/0009-0009-9727-9178
Aisha Ejura DAHUNSI: https://orcid.org/0000-0002-1513-1631
Research Article
World Journal of Advanced Pharmaceutical and Life Sciences, 2026, 11(01), 093–098.
Article DOI: 10.53346/wjapls.2026.11.1.0018
Publication history:
Received on 15 August 2026; revised on 20 September 2026; accepted on 22 September 2026
Abstract:
This study was carried out by collecting fifty Pandinus imperator Scorpions ascetically extracting the venom and tested on selected bacteria. The extracted venom was in crude (non-lyophilized) state but lyophilized to compare their different efficacy and was passed through a membrane filter. The Bacteria (Pseudomonas aeruginosa, Escherichia coli, and Acinetobacte aumannii) were isolated from drinking water sources of the University of Abuja after which it was preserved in slant for further use. Appropriate differential and selective agar were used to culture and sub-culture the isolates and serotyped while Analytical Profile Index (API) kits were used to confirm the isolated bacteria. Column chromatography of the venom to determine the active ingredient was also done after which high-performance liquid chromatography was carried out. The result shows in mean lowest zone for crude venom against isolated bacteria was 3mm at 25% against Acinetobacter baumanni while the highest was 11.5mm at 100% against Pseudomonas aeruginosa, the lowest for crude venom against serotyped was 4mm at 25% agains The lowest MIC was 25% and 20mg/ml while the highest is 50% and 30mg/ml, the lowest MBC was 50% and 30mg/ml while the lowest was 75% and 40mg/ml. Column chromatography and High-Performance Liquid Chromatography were used to determine Pi3 and Pi5 which were found to be the active components in the venom. ANOVA statistical analysis carried out showed no significant difference in the activities of both venom states.
Keywords:
Antimicrobial Peptides; Antibacterial Activity; Emperor Scorpion; Pandinus Imperator; Scorpion Venom; Column Chromatography, High-Performance Liquid Column Chromatography
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