In vitro cytotoxic, thrombolytic, anthelmintic and antioxidant activities of Litsea monopetala: A medicinal plant
1 Department of Applied Chemistry and Chemical Engineering, Noakhali Science and Technology University, Noakhali,-3814, Bangladesh.
2 Department of Public health, North South University, Dhaka-1229, Bangladesh.
3 Department of Pharmacy, Atish Dipankar Science and Technology University, Dhaka-1230, Bangladesh.
4 Department of Food Technology and Nutrition Science, Noakhali Science & Technology University, Noakhali-3814, Bangladesh.
5 Department of Public Health, American International University- Bangladesh, Dhaka-1213, Bangladesh.
6 School of Health Sciences, University of New Haven, Connecticut, USA.
Research Article
World Journal of Chemical and Pharmaceutical Sciences, 2022, 01(01), 018–027.
Article DOI: 10.53346/wjcps.2022.1.1.0024
Publication history:
Received on 18 January 2022; revised on 10 March 2022; accepted on 12 March 2022
Abstract:
The present study was conducted to assess the cytotoxicity, thrombolytic, anthelmintic and antioxidant activity of mehanolic extract of Litsea monopetala (Family: Lauraceae) leaves in laboratory using in vitro methods. Cytotoxicity test was done by brine shrimp lethality bioassay where the extract concentration was 400, 200, 100, 50, 25, 12.5, 6.25, 3.125, 1.5625, 0.78125 (µg/ml). In vitro thrombolytic activity of Litsea monopetala was performed by clot lysis method using extract concentration 2.5, 5, 10 and 20 (mg/mL) in saline water. Anthelmintic activity test was done by using adult earthworms where 10, 20, 40, 60, 80 (mg/ml) extract concentration were used. Finally antioxidant activity was determined by total phenolic content determination using Folin-Ciocalteu reagent. The Litsea monopetala extract showed cytotoxic activity against brine shrimp nauplii and LC50 value was 41.05(µg/ml) and the investigated thrombolytic activity in our research was 9.52, 9.49, 13.64 and 17.50 % respectively as % of clot lysis. The paralysis time were at 76.75 min, 60 min, 51.75 min, 44.5 min and 64.5 min and death were at 90.50min, 63.75min, 55.50min, 44.75min and 71min. respectively. The Litsea monopetala extract displayed significant antioxidant activity which was 20.75 (mg of GAE / gm) of extracts. The activities observed could be attributed to presence of some of the phytochemicals which have been related with cytotoxic, thrombolytic, anthelmintic and antioxidant property.
Keywords:
Litsea monopetala; Cytotoxic; Thrombolytic; Anthelmintic and antioxidant activity
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